Genetic Epidemiology, Translational Neurogenomics, Psychiatric Genetics and Statistical Genetics Laboratories investigate the pattern of disease in families, particularly identical and non-identical twins, to assess the relative importance of genes and environment in a variety of important health problems.
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PMID
18487284
TITLE
Measuring carbohydrate-deficient transferrin by direct immunoassay: factors affecting diagnostic sensitivity for excessive alcohol intake.
ABSTRACT
BACKGROUND NlmCategory: BACKGROUND
carbohydrate-deficient transferrin (CDT) is a marker of alcohol intake that is used for detecting or monitoring alcohol-use disorders. The introduction of a new direct immunoassay for CDT justifies reevaluation of test performance and reexamination of factors affecting test diagnostic sensitivity and specificity.
METHODS NlmCategory: METHODS
individuals enrolled in twin/family studies of alcohol use and dependence provided blood samples and information on recent alcohol use. Serum CDT concentration was measured in 2 088 people with the N Latex CDT (Dade Behring) method, and CDT percentage (CDT%) was calculated as the proportion of the total transferrin concentration measured with Roche reagents.
RESULTS NlmCategory: RESULTS
diagnostic sensitivity was low, both for comparisons of men who reported an alcohol intake of >28 drinks/week vs those who consumed < or = 28 drinks/week (28% sensitivity) and for women who consumed >14 drinks/week vs those who consumed < or = 14 drinks/week (18% sensitivity), at cutoff values that yielded a 95% specificity. Body mass index, variables associated with metabolic syndrome, and smoking had notable effects on the probability of an abnormal CDT result with excessive alcohol use. Diagnostic sensitivity was greater in men of normal weight (43%) than in obese men (10%) and greater in male smokers (38%) than in male nonsmokers (21%). In women, diagnostic sensitivities were < or = 20%, even for those of normal weight and for smokers.
CONCLUSIONS NlmCategory: CONCLUSIONS
CDT is a poor marker of excessive alcohol intake in both women and men who are overweight or obese. It is also less useful in nonsmokers than in smokers. The diagnostic performance of the direct immunoassay and the effects of obesity and smoking are similar to those reported with previous anion-exchange immunoassay methods.
DATE PUBLISHED
2008 Jul
HISTORY
PUBSTATUS PUBSTATUSDATE
aheadofprint 2008/05/16
pubmed 2008/05/20 09:00
medline 2008/08/09 09:00
entrez 2008/05/20 09:00
AUTHORS
NAME COLLECTIVENAME LASTNAME FORENAME INITIALS AFFILIATION AFFILIATIONINFO
Whitfield JB Whitfield John B JB Genetic Epidemiology Unit, Queensland Institute of Medical Research, Brisbane, Australia. John.Whitfield@qimr.edu.au
Dy V Dy Veronica V
Madden PA Madden Pamela A F PA
Heath AC Heath Andrew C AC
Martin NG Martin Nicholas G NG
Montgomery GW Montgomery Grant W GW
INVESTIGATORS
JOURNAL
VOLUME: 54
ISSUE: 7
TITLE: Clinical chemistry
ISOABBREVIATION: Clin. Chem.
YEAR: 2008
MONTH: Jul
DAY:
MEDLINEDATE:
SEASON:
CITEDMEDIUM: Print
ISSN: 0009-9147
ISSNTYPE: Print
MEDLINE JOURNAL
MEDLINETA: Clin Chem
COUNTRY: United States
ISSNLINKING: 0009-9147
NLMUNIQUEID: 9421549
PUBLICATION TYPE
PUBLICATIONTYPE TEXT
Journal Article
Research Support, N.I.H., Extramural
COMMENTS AND CORRECTIONS
GRANTS
GRANTID AGENCY COUNTRY
AA007535 NIAAA NIH HHS United States
AA007728 NIAAA NIH HHS United States
AA011998 NIAAA NIH HHS United States
AA013321 NIAAA NIH HHS United States
AA013326 NIAAA NIH HHS United States
AA014041 NIAAA NIH HHS United States
GENERAL NOTE
KEYWORDS
MESH HEADINGS
DESCRIPTORNAME QUALIFIERNAME
Adolescent
Adult
Aged
Aged, 80 and over
Alcohol Drinking
Alcoholism diagnosis
Biological Markers blood
Body Mass Index blood
Butyrylcholinesterase blood
Cholesterol, HDL blood
Cholesterol, LDL blood
Female blood
Ferritins blood
Humans blood
Immunoassay blood
Male blood
Middle Aged blood
ROC Curve blood
Reference Values blood
Sensitivity and Specificity blood
Smoking blood
Transferrin analysis
Triglycerides blood
SUPPLEMENTARY MESH
GENE SYMBOLS
CHEMICALS
REGISTRYNUMBER NAMEOFSUBSTANCE
0 Biological Markers
0 Cholesterol, HDL
0 Cholesterol, LDL
0 Transferrin
0 Triglycerides
0 carbohydrate-deficient transferrin
9007-73-2 Ferritins
EC 3.1.1.- Butyrylcholinesterase
OTHER ID's